Awakening sleeping beauty: production of propionic acid in
PA production in E. coli expressing the operon [18,19,20,21,22] indicates that ScpA, ScpB and ScpC are sufficient to form the cycle (Fig. 1, red).Indeed, in studying the kinetics of the enzymes in vitro, Haller et al. coupled ScpA (formerly Sbm) to ScpB and observed the two-step conversion of succinyl-CoA into propionyl-CoA [].However, this …
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